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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200511132
Wong et al.
• Figure S1 HURP depletion in Hct116 generates unaligned chromosomes and an increase in mitotic index.
• Video 1 Unaligned chromosomes persist in HURP-depleted metaphase cells.
• Video 2 HURP-depleted metaphase cells have kinetochores under partial tension at metaphase.
• Video 3 HURP-depleted cells are delayed in mitotic progression.
• Video 4 HURP-depleted, ch-TOG-depleted, and nocodazole-treated HeLa cells initiate anaphase in the presence of unaligned chromosomes.
• Video 5 HURP-depleted cells initiate anaphase with kinetochores under partial tension.
• Video 6 HURP-depleted cells have less stable spindles when treated with nocodazole.
• Video 7 Repolymerization of microtubules was delayed in HURP-depleted cells upon release from nocodazole treatment.
• Video 8 Reformation of a bipolar spindle was delayed in HURP-depleted cells upon release from nocodazole treatment.
• Video 9 HURP decreases the turnover rate of α/β-tubulin on the mitotic spindle.
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