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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200601075
Cameron et al.
• Figure S1 Methods demonstrated: speckle tracking, cumulative velocity histogram, and kymograph of photoactivation mark.
• Figure S2 Localization of Kif2a and effects of p150_CC1 injection in PtK1 cells.
• Table S1 Summary of MT poleward flux measurements with different methods
• Video 1 The poleward movement of speckles relative to kinetochores and poles in a PtK1 control bipolar spindle.
• Video 2 A photoactivated mark of tubulin fluorescence moving to the pole of a control spindle in a PtK1 cell expressing PA-GFP-tubulin.
• Video 3 Four photoactivation simulations described in the supplemental text.
• Video 4 Poleward flux of tubulin speckles and kinetochores in a PtK1 cell treated with 200 µM monastrol.
• Video 5 A photoactivated mark moving to the pole in a PtK1 cell expressing PA-GFP-tubulin treated with 200 µM monastrol for 30 min.
• Video 6 Poleward flux of speckles relative to kinetochores in a monopolar PtK1 cell treated with 100 µM monastrol.
• Video 7 GFP-EB1 comets moving away from the pole on the ends of polymerizing MTs in a PtK1 cell expressing GFP-EB1 treated with 200 µM monastrol.
• Video 8 A photoactivated mark moving to the pole in a monopolar PtK1 cell expressing PA-GFP-tubulin treated with 200 µM monastrol.
• Video 9 Rhodamine-tubulin fluorescence in a p150_CC1-injected PtK1 cell.
• Supplemental text (PDF)
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