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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200608128
Kim et al.

Figure S1 Depletion of cytoplasmic dynein or kinesin stops peroxisome movement.

Figure S2 Depletion of one motor (kinesin or dynein) does not deplete the other motor of opposite polarity in both total cell extracts and the organelle fraction.

Figure S3 Kinesin can interact with dynein but not with dynactin.

Figure S4 Deletion of the microtubule-binding domain of p150glued does not affect the transport of organelles along microtubules.

Video 1 EGFP-p150glued decorates tips and the length of microtubules.

Video 2 EGFP-p150glued decorates tips and the length of microtubules.

Video 3 EGFP-ΔN-p150glued is distributed randomly in the cytoplasm.

Video 4 Movement of EGFP-tagged peroxisomes in the processes of an S2 cell treated with cytochalasin D.

Video 5 Movement of EGFP-tagged peroxisomes in the processes of an S2 cell expressing mRFP-p150glued.

Video 6 Movement of EGFP-tagged peroxisomes in the processes of an S2 cell expressing the truncation mutant of p150glued, mRFP-ΔN-p150glued.

Table S1 Effects of motor depletion and p150 truncation on the movement of organelles in S2 cells





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