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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200701065
Maddox et al.
• Figure S1 KNL-2 function is required throughout development and is not restricted to the first mitotic division of the C. elegans embryo.
• Figure S2 KNL-2 makes an equivalent contribution to CeCENP-A in condensation and partially colocalizes with CeCENP-A in interphase nuclei of C. elegans embryos.
• Figure S3 HsKNL2 is required for mitotic chromosome segregation.
• Video 1 Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-histone and GFP-γ-tubulin (strain TH32).
• Video 2 Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo that has been depleted of CeCENP-A by RNAi.
• Video 3 Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo that has been depleted of KNL-2 by RNAi.
• Video 4 Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2.
• Video 5 Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in which CeCENP-A has been depleted by RNAi.
• Video 6 Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-CeCENP-A.
• Video 7 Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-CeCENP-A in which KNL-2 has been depleted by RNAi.
• Video 8 Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in which CeCENP-C was depleted by RNAi.
• Video 9 Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 (strain OD31) in which KNL-1 has been depleted by RNAi.
• Video 10 Spinning disk confocal time-lapse imaging of mitosis in asynchronous HeLa cells transfected with control, nontargeting siRNA oligonucleotides, or siRNA oligonucleotides targeting HsKNL2.
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