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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200703002
Muhlhausser et al.
• Figure S1 Time course of changes in NE permeability and nucleoporin disassembly during the early phase of in vitro NEBD.
• Figure S2 Nuclear accumulation of a fluorescent BSA-NLS conjugate is blocked by Impβ45-462, RanQ69L(GTP), RanT24N, and an excess of unlabeled BSA-NLS and is inhibited by RCC1.
• Figure S3 RanQ69L does not interfere with the initial steps of NPC disassembly in mitotic egg extracts.
• Figure S4 Excess BSA-NLS conjugates or recombinant p50/dynamitin inhibit the late steps of NEBD in vitro.
• Figure S5 Effect of RanT24N and RanQ69L on microtubule polymerization around nuclei during NEBD in vitro.
• Video 1 Time-lapse video of semipermeabilized GFP-LAP2β HeLa cells incubated in interphase Xenopus egg extract supplemented with a 155-kD TRITC-labeled dextran.
• Video 2 Time-lapse video of semipermeabilized GFP-LAP2β HeLa cells incubated in mitotic CSF-arrested Xenopus egg extract supplemented with a 155-kD TRITC-labeled dextran.
• Video 3 Time-lapse video of semipermeabilized GFP-LAP2β HeLa cells incubated in mitotic CSF-arrested Xenopus egg extract supplemented with a 155-kD TRITC-labeled dextran in the presence of the CDK1 inhibitor alsterpaullone.
• Video 4 Time-lapse video of semipermeabilized GFP-LAP2β HeLa cells pretreated with 15 µM recombinant Impβ45-462 incubated in mitotic CSF-arrested Xenopus egg extract supplemented with a 155-kD TRITC-labeled dextran.
• Video 5 Time-lapse video of semipermeabilized GFP-LAP2β HeLa cells incubated in mitotic CSF-arrested Xenopus egg extract supplemented with a 155-kD TRITC-labeled dextran in the presence of 20 µM of recombinant RanQ69L(GTP).
• Video 6 Time-lapse video of semipermeabilized GFP-LAP2β HeLa cells incubated in mitotic CSF-arrested Xenopus egg extract supplemented with a 155-kD TRITC-labeled dextran in the presence of recombinant RCC1.
• Video 7 Time-lapse video of semipermeabilized GFP-LAP2β HeLa cells preloaded with RanT24N and incubated in mitotic CSF-arrested Xenopus egg extract supplemented with a 155-kD TRITC-labeled dextran in the presence of 20 µM RanT24N.
• Video 8 Time-lapse video of semipermeabilized GFP-LAP2β HeLa cells incubated in mitotic CSF-arrested Xenopus egg extract supplemented with a 155-kD TRITC-labeled dextran in the presence of 10 µM nocodazole.
• Video 9 Time-lapse video of HeLa cells (expressing GFP-LAP2β, H2B-mRFP, and mPlum-GST-M9) progressing into mitosis.
• Video 10 Time-lapse video of nocodazole-treated HeLa cells (expressing GFP-LAP2β, H2B-mRFP, and mPlum-GST-M9) progressing into mitosis.
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