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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200706206
van Rheenen et al.

Figure S1 FRET-based PIP2 hydrolysis assay.

Figure S2 Micell pre-absorption of monoclonal anti-PI(4,5)P2 demonstrates specificity to PI(4,5)P2.

Figure S3 Relative intensity changes in images do not change the Pearson's correlation coefficient.

Figure S4 FRET controls for GFP-cofilin FRET.

Figure S5 Rapamycin-induced translocation of a 5-ptase domain to the PM resulting in a reduction of PIP2.

Video 1 Rampamycin-induced PIP2 reduction leads to the formation of membrane protrusions.





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