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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200711053
Goshima et al.
• Figure S1 Characterization of anti-Dgt antibodies, codepletion of Dgt proteins, and Dgt localization.
• Figure S2 Identical RNAi phenotypes of Dgt2-6, Dgrip71, and Dgrip75.
• Figure S3 Chromosome misalignment and kMT defects after Dgt RNAi.
• Video 1 FRAP of GFP-Dgt5 on the spindle.
• Video 2 Spindle defects after RNAi of Dgt2–6, Dgrip71, 75 (γ-TuRC), or Dgrip84 (γ-TuSC)
• Video 3 Severe spindle defects after double RNAi of Cnn and Dgt2-6, Dgrip71, 75 (γ-TuRC), or Dgrip84 (γ-TuSC).
• Video 4 FRAP of GFP-tubulin after Dgt5 and Cnn RNAi FRAP of GFP-tubulin in the metaphase spindle.
• Video 5 Delay of chromosome alignment and anaphase onset after Dgt6 RNAi.
• Video 6 Abnormal spindle morphology and severe chromosome misalignment after double Cnn/Dgt6 RNAi.
• Video 7 Kinetochore MT bundles are defective after RNAi of double Cnn/Dgt5.
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