JCB Annotated
Video Index
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| Mitosis |
A motor helps holocentrics pull away from poles
Kinetochores switch to depolymerization at anaphase
Merotelic attachments are a major source of segregation errors
Dynein shuts down the spindle checkpoint
Kinetochores may act as sites for catalytically sequestering checkpoint proteins
Microtubule ejection thins out spindles
Dynein takes depolymerization to the poles
Kinetochore-nucleated microtubules help build spindles
Interphase microtubules are dragged into the spindle region during prophase
EB1-driven dynamics helps during spindle construction
Aurora-A is required for spindle assembly
Evidence for a possible spindle matrix
Skeletor as a candidate component of a spindle matrix
Mitochondrial replication factories
Myosin V may help partition the ER
| Cytokinesis |
Minimal requirements for inducing cytokinesis
Rho activation during cytokinesis
Rings that promote cytokinesis
Centrosomes are needed for spindle orientation but not cytokinesis
| Nuclear structure |
| Trafficking |
Endosome to trans-Golgi traffic is vesicular
A marker for sequential exocytosis
A myosin V moves yeast secretory vesicles
Rapid cycling of lipid rafts to and from the Golgi
Membrane docking at the immunological synapse requires Rab27a
| Cytoskeleton |
Microtubule and actin movements are coordinated
Actin-dependent picket fences slow diffusion in the plasma membrane
Myosin recruitment drives the distribution of nuclei in fly embryos
A sensor for the activity and abundance of MLCK
MLCK stimulates rapid contraction; Rho kinase stimulates sustained contraction
Tea1p rides on microtubule ends
Microtubule catastrophe under pressure
Dynactin and microtubules search out their organelle targets
Tea1p travels to cell ends and keeps polarity factors anchored there
Cytoplasmic microtubules position the nucleus
Cortex-microtubule interactions position the nucleus and spindle
| Cell Movement |
Determining direction of movement
Cdc42 focuses the direction of movement
Cells can move by either destroying or squeezing through the matrix
Making and remodeling adhesions
Splitting podosomes to make new adhesive contacts
Focal complexes behave differently at the front and back of the cell
HA and CD44 work together to form adhesion-filled protrusions
Arg connects microtubules and actin to help protrusion
Microtubules target adhesions precisely
Microtubules make a persistent push to the front
Released microtubules dictate the direction of cell movement
Kinesin delivers a signal inhibiting adhesion sites
APC is deposited from microtubules at the cell surface to promote outgrowth
A docking structure for transmigrating leukocytes
Tether durations needed for leukocyte attachment and rolling
| Neurite outgrowth |
NCAM traps machinery at new synapses
Actin and microtubule movements are coupled in growth cones
Growth cones grab sites that can withstand tension
PKC increases neurite growth by increasing microtubule growth
| Cell adhesion |
Adhesion is necessary for elongation
| Cell differentiation |
Myo-endothelial progenitors isolated from muscle
Skeletal muscle cells can couple with heart muscle cells
Release of platelets from megakaryocytes
Frog Dishevelled is transported dorsally to specify dorsal fate
| Apoptosis |
Caspase cleavage of GRASP65 helps fragment the Golgi during apoptosis
Apoptotic cells show a transient loss in mitochondrial membrane potential
| Protein degradation and stress responses |
| Signaling |
Mitochondria slow down for calcium
Activated Ras defines the front of chemotaxing cells
| Pathogenesis |
Dynein delivers HIV to the nucleus
Listeria use cellular engulfment machinery to invade neighboring cells
| Organelle biogenesis |
| Extracellular matrix |
Parallel fibropositors make for strong tendons
Calcium inhibition of AC6 allows formation of gaps between endothelial cells
Matrix meshes can be strapped together with small-scale forces